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KMID : 0351619750160010235
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Kyungpook Medical Journal
1975 Volume.16 No. 1 p.235 ~ p.244
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Surfactant Activities of the Lung Extracts and Washings, and Effect of X-Irradiation In Vitro on the Pulmonary Surfactant in Rabbits
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Abstract
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T´he extraction method of preparing the pulmonary surfactant is crucial in determining and evaluating the surfactant activities in situ.
In the prosnt study, two most commonly used methods ~,vere compared: namely, the extracts and washings.
Also, in an attempt to clarify the effect of X-irradiation cn the pulmonary surfactant system, X-ray of 300R, 600R and 1, OOOR, respectively ~i-as irradiated iv the lung extracts and lung washings obtained from the rabbit in Vitro, and the pulmonary surfactant activities as well as the protein contents of both extracts and washings ~i~ere studied at 1, 4, 8, 12 and 24 hours after the irradiation at 20C.
The results obtained are summarized as follows:
1) The maximal and minimal surface tensions, width of the tension-area diagram at the surface area of 40 0, stability index and protein content of the lung washings in the non-irradiated normal group was 44.1 dyne/cm, 8.0 dyne/cm, 14.2 dyne/cm, 1.38 and 115.8 mg/ml, respectively. In the lung extracts, the value was 36.8dyne/cm, 13.6dyne/cm, 9.2dyne/cm, 0.911and 884.7mg/ml, respectively.
2) In the normal group, both the lung washings and lung extracts showed no noticeable change in the pulmonary surfactant activities and protein contents upto 24 experimental hours at 20C.
3) X-irradiation of 300R in Vitro produced no meaningful change in the surfactant activities or protein contents from the control, but 6008 and 1.0008 of X-irradiation showed a tendency of sligt changes in the surfactant activities from the ccnirel with the increased values of the minimal surface tensions.
4) Form the ab3ve, it may be considered that the lung ~rashings are better suited than the lung extracts fcr the measurement of the pulmonary surfactant activities. Also, at 20C, the pulmonary surfactant activities as well as thQ protein contents do not seem to be altered greatly for at least 24 hours. Finally, X-irradiation in Vitro does not seem to produce any noticeable change in the pulmonary surfactant.
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